不同检测方法对粪大肠菌群检测的敏感性探讨
马娟1 ，高正2
（1.陕西省人民医院，陕西 西安，710068；2.西安市安康医院，陕西 西安，710000）
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摘要：
目的 比较酶底物法、多管发酵法和滤膜法对粪大肠菌群检测的敏感性。方法 分别采用多管发酵法、酶底物法和滤膜法对待测水样进行检测，通过精密度试验和结果一致性试验比较三种方法的利弊。结果 精密度试验表明，酶底物法精密度最高，滤膜法次之，多管发酵法精密度最差。结果一致性试验表明，三种方法对粪大肠菌群的检测一致性较高。结论 对粪大肠菌群检测时，实际操作中可根据水样污染程度、精密度要求、经费等方面对三种检测方法进行权衡。
关键词：
中图分类号：R155.5文献标志码：A文章编号：2096-1413(2016)27-0143-02
Exploration on the sensitivity of different detection methods to the detection of fecal flora
MA Juan 1, GAO Zheng 2
(1. Shaanxi Provincial People`s Hospital, Xi`an 710068; 2. Ankang Hospital of Xi`an, Xi`an 710000, China)
ABSTRACT: Objective To compare the sensitivity of the enzyme substrate technique, multi-tube fermentation technique and membrane filtration technique in the detection of fecal flora. Methods The enzyme substrate technique, multi -tube fermentation technique and membrane filtration technique were used to detect the water samples, and the advantages and disadvantages of the three methods were compared through the precision test and the results consistency test. Results The precision experiment showed that the enzyme substrate technique had the highest precision, the membrane filtration technique was the second and the multiple tube fermentation method was the worst. The consistency test of the results showed that the consistency of the three methods was high. Conclusion In practice, a proper method among the three methods should be carried out in the detection of ecal flora according to the pollution degree of water sample, the precision requirements and the funds.
KEYWORDS: fecal flora; multi-tube fermentation technique; membrane filtration technique; enzyme substrate technique
参考文献：
[1] 江磊,朱德军,陈永灿,等.我国地表水体粪大肠菌群污染现状分析 [J].水利水电科技进展,2015,35(3):11-18.
[2] 周彬宇,操家顺,谢坤.膜生物反应器处理传染病医院污水研究[J]. 环境科技,2016,29(1):10-14.
[3] 李宗全,黄芳,许江,等.总大肠菌群/粪大肠菌群检测方法中酶底物法封口技术的改进[J].环境科学导刊,2014,33(z1):90-91.
[4] 朱培瑜,魏轲.固定底物酶底物法与多管发酵法和快速纸片法测定水中粪大肠菌群(耐热大肠菌群)的比较[J].四川环境,2014,33(2):30-33.
[5] 何柳飞.多管发酵法测定粪大肠菌群中的试验室质量控制探讨[J]. 环境,2013(z2):16.
[6] EATON AD,CLESCERI LS,RICE EW,et al.Standard methods for the examination of water and wastewater[M].Washington D.C. Amer Public Health Assn,2005.
[7] 宋雪英,伦小文,李玉双,等.太子河流域水体中总大肠菌群污染现 状研究[J].安徽农业科学,2010,38(28):15790-15791.
[8] 陈爱朝,李碧科.紫外消毒后出水粪大肠菌群数的检测研究[J].中国给水排水,2015,31(6):97-98.
[9] 高凡,蒋懿.粪大肠菌群检测方法探讨[J].吉林水利,2010,5(336):98-99.
[10] 安淼.粪大肠菌群检测方法研究[J].农业科技与信息,2016,2(475):45-46.