骨量减少患者血清中外泌体microRNA标志物的筛选

杜昱和1 ，2 ，李恒1 ，郝文庆1 ，2 ，杨文娟1，2 ，田竹芳1

（1.西安市中心医院内分泌科，陕西 西安，710003；2.延安大学医学院，陕西 延安，716000）

浏览次数：241次 下载次数：383次

摘要：

目的 筛选出用于早期预测骨量减少的血清外泌体microRNA 的标志物。方法 收集本院内分泌科2016年1月至2017年1月原发性骨量减少患者10例（A 组）、正常围绝经期及老年女性4例（B组）。每组选取3例患者血清，检测其外泌体中的 miRNAs表达情况并进行组间比较，筛选出差异倍数较大、表达量较高、既往文献中已证实与骨量减少有关的miRNAs 标志物。结果 筛选出与骨量减少有关的miRNAs共34个，其中上调的miRNAs共28个，下调的miRNAs共6个。结论 检测出 的34个miRNAs可反映骨量减少的病情。

关键词：外泌体；microRNA；骨量减少；标志物

中图分类号：R58文献标志码：A文章编号：2096-1413(2017)20-0001-03

The screening of serum exosomal microRNA biomarkers for osteopenia
DU Yu-he 1, 2, LI Heng 1, HAO Wen-qing 1, 2, YANG Wen-juan 1, 2, TIAN Zhu-fang 1
(1. Department of Endocrinology, Xi``an Central Hospital, Xi``an 710003; 2. Medical College of Yan``an University, Yan``an 716000, China)

ABSTRACT：Objective To screen out the biomarkers of serum exosomal microRNA in early prediction of osteopenia. Methods Ten patients with primary osteopenia (group A) and four normal perimenopausal or elderly women (group B) were collected from the department of endocrinology in our hospital from January 2016 to January 2017. Three patients in each group were selected to detect the expression of miRNA in serum, and the results were compared between the two groups, then the miRNAs biomarkers were screened out, which showed that the difference was larger, the expression was higher and has been identified to be associated with osteoporosis in previous literature. Results Thirty-four miRNAs related to osteoporosis were identified, 28 miRNAs were up-regulated, and 6 miRNAs were down-regulated. Conclusion Detected 34 miRNAs could reflect the condition of osteopenia.
KEYWORDS: exosome; microRNA; osteopenia; biomarkers

参考文献：
[1] 中华医学会骨质疏松和骨矿盐疾病分会.原发性骨质疏松症诊治指南(2011年)[J].中华骨质疏松和骨矿盐疾病杂志,2011,4(1):2-17.
[2] SEOK HY,HAM JY,JANG ES,et al.MicroRNA target recognition: insights from transcriptome -wide non -canonical interactions [J].Mol Cell, 2016,39(5):375-381.
[3] CHEN Q,LIU WB,SINHA KM,et al.Identification and characterization of microRNAs controlled by the osteoblast -specific transcription factor osterix[J].Plos One,2013,8(3):e58104.
[4] KUREEL J,DIXIT M,TYAGI AM,et al.miR-542-3p suppresses osteoblast cell proliferation and differentiation, targets BMP -7 signaling and inhibits bone formation[J].Cell Death Dis,2013,5(2):e1050.
[5] NG EK,CHONG WW,JIN H,et al.Differential expression of microRNAs in plasma of patients with colorectal cancer: a potential marker for colorectal cancer screening[J].Gut,2009,58(10):1375-1378.
[6] ZAMPETAKI A,KIECH S,DROZDOV I,et al.Plasma microRNA profiling reveals loss of endothelial miR-126 and other microRNAs in type 2 diabetes[J].Circ Res,2010,107(6):810-817
[7] GALLO A,TANDON M,ALEVIZOS I,et al.The majority of microRNAs detectable in serum and saliva is concentrated in exosomes[J].Plos One,2011,7(3):e30679.
[8] HUNTER MP,ISMAIL N,ZHANG X,et al.Detection of microRNA expression in human peripheral blood microvesicles[J].Plos one,2008,3(11): e3694.
[9] CHEVILLET JR,KANG Q,RUF IK,et al.Quantitative and stoichiometric analysis of the microRNA content of exosomes[J].Proc Natl Acad Sci USA,2014,111(41):14888-14893.
[10] SUN W,ZHAO C,LI Y,et al.Osteoclast-derived microRNA-containing exosomes selectively inhibit osteoblast activity[J].Cell discov,2016,2 (4):16015.
[11] LI D,JIN L,GUO B,et al.Osteoclast-derived exosomal miR-214-3p inhibits osteoblastic bone formation[J].Nat Commun,2016(7):10872.
[12] ZHAO J,WANG C,SONG Y.Arsenic trioxide and microRNA -204 display contrary effects on regulating adipogenic and osteogenic differentiation of mesenchymal stem cells in aplastic anemia[J].Acta biochim biophys Sin (Shanghai),2014,46(10):885-893.
[13] IACULLI F,FILIPPO ESD,PIATTELLI A,et al.Dental pulp stem cells grown on dental implant titanium surfaces: an in vitro evaluation of differentiation and microRNAs expression [J].J Biomed Mater Res B Appl Biomate,2016,6(1):26-28.
[14] SUGATANI T,HRUSKA KA.Impaired micro-RNA pathways diminish osteoclast differentiation and function [J].J Biol Chem,2009,284 (7): 4667-4678.
[15] WEI J,SHI Y,ZHENG L,et al.Karsenty, miR -4s inhibit osteoblast proliferation and differentiation in the mouse by targeting SATB2 [J].J Cell Biol,2012,197(4):509-521.
[16] KANG X,KANG F,YANG B,et al.miRNA-144 inhibiting osteogenesis of mesenchymal stem cells by targeting cadherin``41[J].J Third Mil Med Univ,2013,35(10):922-926.
[17] TROMPETER HI,DREESEN J,HERMANN E,et al.MicroRNAs miR-26a, miR-26b, and miR-29b accelerate osteogenic differentiation of unrestricted somatic stem cells from human cord blood[J].BMC Genomics, 2013,14(1):111.
[18] IVAN M,HUANG X.miR-210: fine-tuning the hypoxic response[J]. Adv Exp Med Biol,2014(772):205-227.
[19] HU W,YE Y,ZHANG W,et al.miR-142-3p promotes osteoblast differentiation by modulating Wnt signaling [J].Mol Med Rep,2013,7 (2): 689-693.