桂枝茯苓胶囊及其成分对LPS诱导RAW264.7细胞产生炎症介质的影响

唐兴梅1，李芳1 , 2，刘莉娜1 , 2 ，朱文灯1 ，高晓艳1 ，凌娅1 , 2

（1.江苏康缘药业股份有限公司，江苏 连云港，222001；2.中药制药过程新技术国家重点实验室，江苏 连云港，222001）

浏览次数：132次 下载次数：420次

摘要：

目的 研究桂枝茯苓胶囊及其成分的抗炎活性，并对其抗炎机制进行初步探讨。方法 采用LPS刺激RAW264.7细胞建 立体外炎症模型，用Griess法检测细胞内NO的释放；ELISA法检测桂枝茯苓胶囊及其成分对PGE2、IL-1β、IL-6 和TNF-α分泌的影响；Western Blot法检测桂枝茯苓胶囊及其成分对iNOS和COX-2表达的影响。结果 桂枝茯苓胶囊及其成分能有效抑制LPS刺激下RAW264.7 细胞释放炎症介质NO和PGE2；降低细胞因子IL-1β、IL-6 和TNF-α 的分泌；Western blot 结果表明桂枝茯苓胶囊及其成分能够下调iNOS 和COX-2 蛋白表达水平。结论 桂枝茯苓胶囊及其成分通过抑制iNOS和COX-2的表达进而抑制NO 和PGE2 的分泌，同时抑制炎症因子IL-1β、IL-6 和TNF-α的产生来发挥抗炎作用。

关键词：桂枝茯苓胶囊；RAW264.7细胞；细胞因子；抗炎活性；立体外炎症模型；炎症介质

中图分类号：R285文献标志码：A文章编号：2096-1413(2017)20-0006-03

Influence of releasing inflammatory mediators in the LPS -induced RAW264.7 cells by Guizhi Fuling

capsule and its ingredients
TANG Xing-mei 1, LI Fang 1, 2, LIU Li-na 1, 2, ZHU Wen-deng 1, 2, GAO Xiao-yan 1, LING Ya 1, 2
(1. Jiangsu Kanion Parmaceutical CO., LTD., Lianyungang 222001; 2. State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang 222001, China)

ABSTRACT: Objective To study the anti -inflammatory activity of Guizhi Fuling capsule and its ingredients, and to preliminary explore its anti-inflammatory mechanism. Methods Vitro inflammation model was established by using LPS to stimulate RAW264.7 macrophages. Griess reaction was used for assaying the concentration of intra -cellular NO, and ELISA method was performed to measure the expression of PGE2、IL -1β、IL -6 and TNF -α caused by Guizhi Fuling capsule and its ingredients. The expression of iNOS and COX-2 caused by Guizhi Fuling capsule and its ingredients were evaluated by Western Blot. Results Guizhi Fuling capsule and its ingredients remarkably inhibited the release of NO and PGE2, and reduced the secretion of cytokines IL-1 beta, IL-6 and TNF- alpha. Western blot results showed that Guizhi Fuling capsule and its components could down-regulate the expression levels of iNOS and COX-2 protein. Conclusion Guizhi Fuling capsule and its ingredients inhibite NO and PGE2 secretion by inhibiting the expression of iNOS and COX-2, and also inhibited the secretion of IL-1β、IL-6 and TNF-α.

KEYWORDS: Guizhi Fuling capsule; RAW264.7 cell; cytokine; anti-inflammatory; vitro inflammation model; inflammatory mediator

参考文献：
[1] 李天容.桂枝茯苓胶嚢治疗原发性痛经的临床研究[D].成都:成都中医药大学,2012.
[2] 左志燕.桂枝茯苓胶囊临床应用研究的文献分析[J].中医药导报,2005,11(11):82-83.
[3] 苏真真,李娜,曹亮,等.桂枝茯苓胶囊主要药理作用及临床应用研究进展[J].中国中药杂志,2015,40(6):989-992.
[4] 李青旺,胡建宏.动物生殖免疫学[M].咸阳:西北农林科技大学出版社,2011:85-86.
[5] GR NE A.Keratinocytes and cytokines[J].Vet Immunol Immunopathol, 2002,88(1-2):1-12.
[6] MAZZA J,ROSSI A,WEINBERG JM.Innovative uses of tumor necrosis factor alpha inhibitors[J].Dermatol Clin,2010,28(3):559-575.
[7] 杨晓露,刘朵,卞卡,等.甘草总黄酮及其成分体外抗炎活性剂机制研究[J].中国中药杂志,2013,38(1):99-104.
[8] 郭姗姗.多甲氧基黄酮的抗炎活性及相关分子机制研究[D].青岛:中国海洋大学,2012.
[9] 李楚文.广藿香地下部分乙醇提取物化学成分分析及抗炎活性评价[D].广州:广州中医药大学,2014:22.
[10] 喻婉莹,阚伟娟,于鹏霞,等.青蒿素和二氢青蒿素的抗炎作用及机制[J].中国中药杂志,2012,37(17):2618-2621.
[11] 潘一峰,杨晓露,刘朵,等.防己茯苓汤抗炎组分筛选及其机制研究[J].中成药,2013,35(1):50-54.