GFAP在脊髓损伤后表达的数量—时空关系及其临床意义

马敏杰，王文涛，蒋勇，刘团江，郭华，郝定均

（西安交通大学医学院附属红会医院脊柱外科，陕西 西安，7100554）

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摘要：

目的 观察大鼠脊髓损伤后损伤区胶质纤维酸性蛋白（GFAP）表达量变化的时间及空间分布规律，探讨抑制GFAP增加的最优时间及部位。方法 选取成年SD大鼠60只，随机分为6组，每组10只，各损伤组使用NYU impactor打击器制作大 鼠脊髓损伤模型。分别于造模前、后相应时间节点对各组大鼠实施运动功能BBB评分，于预定时间点处死大鼠，取损伤段脊髓组织行免疫荧光染色，行阳性细胞计数，采用方差分析进行组间比较。结果 造模前后各组大鼠BBB评分比较，差异有显著统计学意义（P<0.01）；各组脊髓灰、白质中均有GFAP表达，但损伤各组GFAP平均阳性细胞数与假手术组相比，均有明显差 异（P<0.01），且以损伤后14d组的表达数量上升最为显著（P<0.01），各组白质区GFAP平均阳性细胞数的增加均较灰质区 更为显著（P<0.01）。结论 脊髓损伤后损伤脊髓组织中GFAP的表达增加，损伤后l4d达到最高，主要集中在损伤白质区。对GFAP进行特异性抑制最好在损伤后l4d以内的脊髓白质区实施。

关键词：脊髓损伤；胶质纤维酸性蛋白；阳性细胞计数

中图分类号：R651.2文献标志码：A文章编号：2096-1413(2017)06-0004-03

The dynamic change of GFAP and its clinical significance after spinal cord injury
MA Min-jie, WANG Wen-tao, JIANG Yong, LIU Tuan-jiang, GUO Hua, HAO Ding-jun
(Department of Spinal Surgery, Honghui Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710054, China)

ABSTRACT: Objective To observe the dynamic change of GFAP after spinal cord injury, especially the changes in different region and on different time point, so as to choose the optimization therapeutic region and time of spinal cord during the inhibition therapy of GFAP. Methods A total of 60 Sprague Dawley rats were randomly assigned into 6 groups, with 10 rats in each group. Except the sham operation group, spinal cord injury models were created by NYU impactor. All rats were assessed in an open field environment by using the Basso-Beattie-Bresnahan (BBB) locomotor rating scale before the perfusing and sampling, and then immunohistochemistry was used to evaluate the level of GFAP in the injuried region. Results There were significant differences of BBB scores between the sham operation group and the model groups (P<0.01).According to the results of immunohistochemistry, GFAP-positive cells were detected in the white matter region and gray matter of model groups, and significant difference was detected between the model group and sham operation group (P<0.01).Compared with the sham operation group, the level of GFAP of 14-day-after-impact group has a great increase in astrocyte cells around the white matter of injury site following spinal cord contusion (P<0.01). Conclusion The most significant increase in the number of GFAP-positive cells was found on the 14-day in the white matter region following impact, so the GFAP-inhibitor should be used in early stage after spinal cord injury and the targeting region is the white matter of injury site.
KEYWORDS: spinal cord injury; glial fibrillary acidic protein; GFAP-positive cells

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