COX-2、bFGF及TGFβ1在急性白血病中的表达及其临床意义

王晓军，茹甫毅，吴双有，朱卫民，田培军

（安康市中心医院，陕西 安康, 725000）

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摘要：

目的 检测不同类型急性白血病（AL）患者在不同阶段的COX-2、bFGF及TGFβ1指标的表达变化情况，分析各指标在 AL的诊断、治疗及预后判断中的临床意义。方法 以在安康市中心医院就诊的90例为AL患者研究对象，该院健康体检者40例为对照组，采用ELISA法进行检测，并进行治疗前、后两个阶段的动态。结果 ① 初诊时，AL组血清COX-2及bFGF水平较 对照组明显升高，而TGFβ1明显降低（P<0.001）；②治疗前ALM组血清 bFGF、COX-2及TGFβ1水平与ALL组患者比较，无显著差异（P>0.05）；③CR（完全缓解）时，与治疗前比较bFGF与COX-2水平明显下降，TGFβ1显著升高（P<0.001）；④治疗前血清bFGF 及COX2水平与骨髓原始及幼稚细胞数呈正相关，TGFβ1 与之呈负相关，bFGF、COX2 的水平均与TGFβ1呈负相 关。结论 COX-2 及bFGF水平显著增加，CR时显著下降，而血清TGFβ1明显减低，CR时明显升高，提示血管生成可能在AL的发生、发展中具有一定作用。因此，动态监测AL患者血清这些因子水平，可作为AL病情评估的重要参考指标。

关键词：急性白血病；环氧合酶-2；碱性成纤维细胞生长因子；转化生长因子

中图分类号：R733.71文献标志码：A文章编号：2096-1413(2017)07-0008-04

Detection and clinical significance of serum COX-2, bFGF, TGFβ1

in acute leukemia
WANG Xiao-jun, RU Fu-yi ,WU Shuang-you , ZHU Wei-min, TIAN Pei-jun

(Ankang City Central Hospital, Ankang 725000, China)

ABSTRACT: Objective To measure the serum levels of COX-2, bFGF, TGFβ1 in patients at different stages of acute leukemia, and discuss the diagnosis, treatment and prognosis of the disease. Methods The serum levels of COX-2, bFGF and TGFβ1 in 90 acute leukemia patients and 40 healthy adults were determined by ELISA, and the results were analyzed at different stages of the disease. The nuclear cells were counted in a bone marrow smear of 90 patients with acute leukemia. Results ①Compared with the normal control group，the serum levels of COX-2 and bFGF were found to beelevated before treatment in the acute leukemia patients, the plasma levels of TGFβ1 were found to be decreased before treatment in the acute leukemia patients (P<0.001); ② There were no differences were found in the levels of COX-2, bFGF and TGFβ1 between the AML group and the ALL group (P>0.05); ③compared with the before treatment,the levels of COX-2, bFGF were significant decreased, the levels of TGFβ1 in the patients with acute leukemia after complete remission (CR) was significant improved (P <0.001); ④there was a positive correlation between the total
number of primary and naive cells and the serum levels of COX-2 and bFGF in the patients with acute leukemia. In addition, a negative correlation was found between the total number of primary and naive cells and the serum level of TGFβ1 in these patients. Moreover, there was a negative correlation was found between the serum levels of COX-2, bFGF and TGFβ1 in the patients with acute leukemia. Conclusion Our study showed the serum levels of angiogenesis-promoting factors COX-2 and bFGF to be elevated before treatment in the acute leukemia patients, but after complete remission, the serum levels of these angiogenic factors were found to be significantly decreased. Further, the serum levels of TGFβ1 were found to decrease before treatment in the acute leukemia patients, but after complete remission, the serum levels were found to be significantly elevated. Angiogenesis may play a role in the leukemogenic process.Therefore, monitoring the levels of serum COX -2, bFGF and TGFβ1 at different stages of acute leukemia can provide clinical evidence of disease progression.
KEYWORDS: acute leukemia; serum COX-2; bFGF;TGFβ1

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